LOCUS       40924_21524        5515 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pGFchs-hph DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5515)
  AUTHORS   Sato M, Kurahashi A, Nishibori K, Fujimori F.
  TITLE     Development of a transformation system for the edible mushroom 
            Grifola frondosa: demonstrating heterologous gene expression and 
            RNAi-mediated gene silencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5515)
  AUTHORS   Sato M, Kurahashi A, Nishibori K, Fujimori F.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2014) Contact:Masayuki Sato Yukiguni Maitake Co. 
            Ltd., Research and Development; 89 Yokawa, Minamiuonuma, Niigata 
            949-6695, Japan URL :http://www.maitake.co.jp
REFERENCE   3  (bases 1 to 5515)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5515)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-FEB-2014) Contact:Masayuki Sato Yukiguni Maitake Co. Ltd., 
            Research and Development; 89 Yokawa, Minamiuonuma, Niigata 949-6695,
            Japan URL :http://www.maitake.co.jp"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5515
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      245..254
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(317..328)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     CDS             1500..2522
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     regulatory      2526..2825
                     /gene="Gf.CHS1"
                     /regulatory_class="terminator"
     gene            2526..2825
                     /gene="Gf.CHS1"
                     /label=Gf.CHS1
     primer_bind     complement(2873..2889)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2897..2913)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2921..2951)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2966..2987)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3275..3863)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4037..4894)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4895..4999)
                     /label=AmpR promoter
     primer_bind     5473..5489
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atgcttggag cggtggagct ggcgcggtcg tggcgctcgg tccttgcaac gtagttggtt
       61 ttttcctatt gaatccgggc ttcttcctga ctgtcttctg gactggcgga ctctgggctc
      121 ggtccggtcc tattctcttc tctccatcgc tggatgagac ggaatgaatc ggcccccggt
      181 cggagatgct tcgtttctcg caccgtggtt tctaagaaaa ggcttcctca gccgcggaaa
      241 tggtccatgg cggcacatat gtctggtgtg aatagcgctt gtcaccagtt gcaggcatgt
      301 gatcagggta cgggtttcgt ccttcgatat ctccgtacgt ggcgctcgcc gcgtgtgtcg
      361 tgtatgtccc tgcgatcgtt gtgcggggtg tccagcgcgt ccattgatat ggtatcgcgc
      421 tagggatcat gcggtgactg acaatcgtgt gcagacgttt cttcgttgca cgagcccgtt
      481 ctttttcgga ccaaggatcc cctgtctctg cccaaggaga gctcttaggt gaggtgctgt
      541 tggagattcg attgagcccc gccttcacca tcgtgggtgc cttcgatcag ggtggcagtg
      601 gcttgtgatc aggaactcga actctgtgag tttgatagcg taacccttcc cctctgccat
      661 taacttcttt gcagcatagg ctacaaatag actactttgg ccgcgtcgag cggcagactc
      721 gatattacgg ctcggtgacg tcgctgctcg gtgatcgcac tcccaaaccg ccaattttca
      781 agtcactagc aatttcgcga tcaaagagct caaccagacg ctgcatgtaa cagaactagt
      841 gccggcccac tgccggccct tggaaatctc agcgtctctg cgcgcggtga cttgactggc
      901 agattgcatc cagcggaaca tgtggactcg cgtgtctgtg ttgtcaacaa tttatatagt
      961 tctctcattg caaggtttcc ttcaatctac gtgggtcaag tgtatcgctt aatatttcgt
     1021 gtgtgtatcg atcagaacag taaggtcatc ggatgcttca agagccgttt gtgactaagt
     1081 atgtggttaa atggtatcaa acctgaagga gcgttttcag tgtactgatg aagtatggtg
     1141 cggctgcatt gcttatcgta attgctcgac aagaagcaaa ggactacgag ggagagctga
     1201 tgaacttata acaccgatga cggtcatgtg atgtggcggc atttaggatt accaaagatg
     1261 gtaaccatat tgatgccgaa tagagctgaa actgcaacaa ggatctatca gagtccatct
     1321 tcagttcagc tcccaccgca gctatcaagg tctgctaaca acgtcctcgt cgttgctcac
     1381 attccctggc ggttttcgtg cacacagaac agaccttcgt tcattttcat atcacactca
     1441 ttcgttttcg ataccccaaa ttgaatcgcg tttcatagcg gccattttgc actcttgcga
     1501 tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca
     1561 gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg
     1621 taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc
     1681 gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg
     1741 gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc
     1801 aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg
     1861 cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa
     1921 tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc
     1981 actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc
     2041 tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct
     2101 ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga
     2161 tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt
     2221 gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc
     2281 ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg
     2341 gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag
     2401 ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct
     2461 gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg
     2521 aatagtcgga tttcttgtac tcaatatgta tgtccggcca ggtttgttct tcgctggttc
     2581 ggcggatata ttgctgggac attgtgtttt tatcgtaatt gtactcgaaa tataccctgc
     2641 ttgatatcgc agtaggtcaa tggtgtgaac tggtgaatct ataagtaatc tcgatcgtgc
     2701 acgtgcgaag accaaagtaa tagtatttcc tagtatttcg cgcttctgcg cgcgttccaa
     2761 ccgtttatgt cataatggac tttggtactt gaggctgagt ctcgagggtt tcattcctat
     2821 ctccagatcc tctagagtcg acctgcaggc atgcaagctt ggcgtaatca tggtcatagc
     2881 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     2941 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     3001 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     3061 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     3121 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     3181 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     3241 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     3301 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     3361 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     3421 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     3481 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3541 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3601 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3661 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     3721 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3781 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3841 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     3901 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     3961 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     4021 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     4081 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     4141 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     4201 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     4261 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     4321 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     4381 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     4441 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     4501 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     4561 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     4621 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     4681 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     4741 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     4801 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     4861 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     4921 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     4981 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     5041 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     5101 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     5161 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     5221 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     5281 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     5341 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     5401 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     5461 agtcacgacg ttgtaaaacg acggccagtg aattcgagct cggtacccgg ggatc
//
